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The immunogenicity of viral haemorragic septicaemia rhabdovirus (VHSV) DNA vaccines can depend on plasmid regulatory sequences


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Título :
The immunogenicity of viral haemorragic septicaemia rhabdovirus (VHSV) DNA vaccines can depend on plasmid regulatory sequences
Autor :
CHICO GRAS, VERONICA  
Ortega-Villaizan, Maria del Mar  
Falco, Antonio  
Tafalla, C.
Perez, L.
Coll, J.M.
Estepa, A.
Editor :
Elsevier
Fecha de publicación:
2022-01
URI :
https://hdl.handle.net/11000/31152
Resumen :
A plasmid DNA encoding the viral hemorrhagic septicaemia virus (VHSV)-G glycoprotein under the control of 5′ upstream sequences (enhancer/promoter sequence plus both non-coding 1st exon and 1st intron sequences) from carp β-actin gene (pAE6-GVHSV) was compared to the vaccine plasmid usually described the gene expression is regulated by the human cytomegalovirus (CMV) immediate-early promoter (pMCV1.4-GVHSV). We observed that these two plasmids produced a markedly different profile in the level and time of expression of the encoded-antigen, and this may have a direct effect upon the intensity and suitability of the in vivo immune response. Thus, fish genetic immunisation assays were carried out to study the immune response of both plasmids. A significantly enhanced specific-antibody response against the viral glycoprotein was found in the fish immunised with pAE6-GVHSV. However, the protective efficacy against VHSV challenge conferred by both plasmids was similar. Later analysis of the transcription profile of a set of representative immune-related genes in the DNA immunized fish suggested that depending on the plasmid-related regulatory sequences controlling its expression, the plasmid might activate distinct patterns of the immune system. All together, the results from this study mainly point out that the selection of a determinate encoded-antigen/vector combination for genetic immunisation is of extraordinary importance in designing optimised DNA vaccines that, when required for inducing protective immune response, could elicit responses biased to antigen-specific antibodies or cytotoxic T cells generation.
Palabras clave/Materias:
VHS virus
DNA vaccine
Promoter
Plasmid
Glycoprotein
Interferon
Fish
Gene expression profile
Área de conocimiento :
CDU: Ciencias puras y naturales: Biología: Biología general y teórica
Tipo documento :
application/pdf
Derechos de acceso:
info:eu-repo/semantics/closedAccess
Attribution-NonCommercial-NoDerivatives 4.0 Internacional
DOI :
https://doi.org/10.1016/j.vaccine.2009.01.103
Aparece en las colecciones:
Instituto de Investigación, Desarrollo e Innovación en Biotecnología Sanitaria de Elche



Creative Commons La licencia se describe como: Atribución-NonComercial-NoDerivada 4.0 Internacional.